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NovaFluor Yellow 700

NovaFluor Yellow 700 (Ex-Max 552 nm/Em-Max 700 nm)

The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Yellow 700 is designed to be discreetly excited by the 561nm laser and has an excitation peak at 552 nm and an emission peak at 700 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
552 nm
Emission maximum
700 nm
Optimal laser line
561
Optimal filter
695/40
Relative brightness
3 of 5
Commercial antibodies
68 from 1 supplier

NovaFluor Yellow 700 has an excitation maximum at 552 nm and an emission maximum at 700 nm, a Stokes shift of 148 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What is NovaFluor Yellow 700?
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Yellow 700 is designed to be discreetly excited by the 561nm laser and has an excitation peak at 552 nm and an emission peak at 700 nm.
What laser excites NovaFluor Yellow 700?
NovaFluor Yellow 700 is optimally excited by the 561 laser line. Its excitation maximum is 552 nm.
What filter should I use to detect NovaFluor Yellow 700?
A 695/40 filter captures the peak of the NovaFluor Yellow 700 emission near 700 nm with minimal spillover.
How bright is NovaFluor Yellow 700?
NovaFluor Yellow 700 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to NovaFluor Yellow 700?
FluoroFinder tracks 68 commercially available NovaFluor Yellow 700 conjugates, searchable by target, clone, host and application.