NovaFluor Yellow 590
NovaFluor Yellow 590 (Ex-Max 552 nm/Em-Max 585 nm)
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Yellow 590 is designed to be discreetly excited by the 561 nm laser and has an excitation peak at 552 nm and an emission peak at 585 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 552 nm
- Emission maximum
- 585 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Commercial antibodies
- 58 from 1 supplier
NovaFluor Yellow 590 has an excitation maximum at 552 nm and an emission maximum at 585 nm, a Stokes shift of 33 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is NovaFluor Yellow 590?
- The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Yellow 590 is designed to be discreetly excited by the 561 nm laser and has an excitation peak at 552 nm and an emission peak at 585 nm.
- What laser excites NovaFluor Yellow 590?
- NovaFluor Yellow 590 is optimally excited by the 561 laser line. Its excitation maximum is 552 nm.
- What filter should I use to detect NovaFluor Yellow 590?
- A 585/42 filter captures the peak of the NovaFluor Yellow 590 emission near 585 nm with minimal spillover.
- How bright is NovaFluor Yellow 590?
- NovaFluor Yellow 590 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to NovaFluor Yellow 590?
- FluoroFinder tracks 58 commercially available NovaFluor Yellow 590 conjugates, searchable by target, clone, host and application.