NovaFluor Red 685
NovaFluor Red 685 (Ex-Max 637 nm/Em-Max 685 nm)
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Red 685 has an excitation peak at 637 nm and an emission peak at 685 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 637 nm
- Emission maximum
- 685 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 3 of 5
- Commercial antibodies
- 87 from 1 supplier
NovaFluor Red 685 has an excitation maximum at 637 nm and an emission maximum at 685 nm, a Stokes shift of 48 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is NovaFluor Red 685?
- The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Red 685 has an excitation peak at 637 nm and an emission peak at 685 nm.
- What laser excites NovaFluor Red 685?
- NovaFluor Red 685 is optimally excited by the 628-640 laser line. Its excitation maximum is 637 nm.
- What filter should I use to detect NovaFluor Red 685?
- A 695/40 filter captures the peak of the NovaFluor Red 685 emission near 685 nm with minimal spillover.
- How bright is NovaFluor Red 685?
- NovaFluor Red 685 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to NovaFluor Red 685?
- FluoroFinder tracks 87 commercially available NovaFluor Red 685 conjugates, searchable by target, clone, host and application.