NovaFluor Blue 555
NovaFluor Blue 555 (Ex-Max 496 nm/Em-Max 555 nm)
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 555 is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 496 nm and an emission peak at 555 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 496 nm
- Emission maximum
- 555 nm
- Optimal laser line
- 488
- Optimal filter
- 575/36
- Relative brightness
- 1 of 5
- Commercial antibodies
- 35 from 1 supplier
NovaFluor Blue 555 has an excitation maximum at 496 nm and an emission maximum at 555 nm, a Stokes shift of 59 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NovaFluor Blue 555?
- The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 555 is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 496 nm and an emission peak at 555 nm.
- What laser excites NovaFluor Blue 555?
- NovaFluor Blue 555 is optimally excited by the 488 laser line. Its excitation maximum is 496 nm.
- What filter should I use to detect NovaFluor Blue 555?
- A 575/36 filter captures the peak of the NovaFluor Blue 555 emission near 555 nm with minimal spillover.
- How bright is NovaFluor Blue 555?
- NovaFluor Blue 555 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to NovaFluor Blue 555?
- FluoroFinder tracks 35 commercially available NovaFluor Blue 555 conjugates, searchable by target, clone, host and application.