Live-or-Dye 615/740
Live-or-Dye™ 615/740 (Ex-Max 612 nm/Em-Max 744 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 615/740 has an excitation peak at 615 nm and an emission peak at 740 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 612 nm
- Emission maximum
- 744 nm
- Optimal laser line
- 628-640
- Optimal filter
- N/A (Spectral)
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 615/740 has an excitation maximum at 612 nm and an emission maximum at 744 nm, a Stokes shift of 132 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Live-or-Dye 615/740?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 615/740 has an excitation peak at 615 nm and an emission peak at 740 nm.
- What laser excites Live-or-Dye 615/740?
- Live-or-Dye 615/740 is optimally excited by the 628-640 laser line. Its excitation maximum is 612 nm.
- What filter should I use to detect Live-or-Dye 615/740?
- A N/A (Spectral) filter captures the peak of the Live-or-Dye 615/740 emission near 744 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 615/740?
- FluoroFinder tracks 2 commercially available Live-or-Dye 615/740 conjugates, searchable by target, clone, host and application.