Live-or-Dye 375/600
Live-or-Dye™ 375/600 (Ex-Max 373 nm/Em-Max 595 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 375/600 has an excitation peak at 375 nm and an emission peak at 600 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 373 nm
- Emission maximum
- 595 nm
- Optimal laser line
- 355
- Optimal filter
- N/A (Spectral)
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 375/600 has an excitation maximum at 373 nm and an emission maximum at 595 nm, a Stokes shift of 222 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Live-or-Dye 375/600?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 375/600 has an excitation peak at 375 nm and an emission peak at 600 nm.
- What laser excites Live-or-Dye 375/600?
- Live-or-Dye 375/600 is optimally excited by the 355 laser line. Its excitation maximum is 373 nm.
- What filter should I use to detect Live-or-Dye 375/600?
- A N/A (Spectral) filter captures the peak of the Live-or-Dye 375/600 emission near 595 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 375/600?
- FluoroFinder tracks 2 commercially available Live-or-Dye 375/600 conjugates, searchable by target, clone, host and application.