AccuOrange
AccuOrange (Ex-Max 480 nm/Em-Max 598 nm)
AccuOrange from Biotium is a reagent used in DNA/RNA quantitation using a microplate assay. It has an excitation peak at 480 nm and an emission peak at 598 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 480 nm
- Emission maximum
- 598 nm
- Optimal laser line
- 488
- Optimal filter
- 585/42
AccuOrange has an excitation maximum at 480 nm and an emission maximum at 598 nm, a Stokes shift of 118 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is AccuOrange?
- AccuOrange from Biotium is a reagent used in DNA/RNA quantitation using a microplate assay. It has an excitation peak at 480 nm and an emission peak at 598 nm.
- What laser excites AccuOrange?
- AccuOrange is optimally excited by the 488 laser line. Its excitation maximum is 480 nm.
- What filter should I use to detect AccuOrange?
- A 585/42 filter captures the peak of the AccuOrange emission near 598 nm with minimal spillover.