RPE-CF647T
RPE-CF®647T (Ex-Max 564 nm/Em-Max 673 nm)
RPE-CF®647T is a tandem fluorophore between R-PE (donor) and CF®647T (acceptor) that can be excited off the 488, 532 or 561 nm laser and has an emission peak at 673 nm. It can be used with the following analysis platforms: Flow Cytometry. Spectrally it is equivalent to PE-Cy5 and PE-Alexa 647.
Excitation & emission spectra
Key facts
- Excitation maximum
- 564 nm
- Emission maximum
- 673 nm
- Optimal laser line
- 488-561
- Optimal filter
- 660/20
- Relative brightness
- 5 of 5
RPE-CF647T has an excitation maximum at 564 nm and an emission maximum at 673 nm, a Stokes shift of 109 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is RPE-CF647T?
- RPE-CF®647T is a tandem fluorophore between R-PE (donor) and CF®647T (acceptor) that can be excited off the 488, 532 or 561 nm laser and has an emission peak at 673 nm. It can be used with the following analysis platforms: Flow Cytometry. Spectrally it is equivalent to PE-Cy5 and PE-Alexa 647.
- What laser excites RPE-CF647T?
- RPE-CF647T is optimally excited by the 488-561 laser line. Its excitation maximum is 564 nm.
- What filter should I use to detect RPE-CF647T?
- A 660/20 filter captures the peak of the RPE-CF647T emission near 673 nm with minimal spillover.
- How bright is RPE-CF647T?
- RPE-CF647T has a relative brightness of 5 out of 5.