CF700
CF®700 (Ex-Max 695 nm/Em-Max 720 nm)
CF®700 from Biotium is a fluorophore with an excitation peak at 695 nm and an emission peak at 720 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 700 and Cy5.5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 695 nm
- Emission maximum
- 720 nm
- Optimal laser line
- 628-640
- Optimal filter
- 710/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 240000
- Molecular weight
- 2315
- Commercial antibodies
- 40 from 2 suppliers
CF700 has an excitation maximum at 695 nm and an emission maximum at 720 nm, a Stokes shift of 25 nm. Its extinction coefficient is 240000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CF700?
- CF®700 from Biotium is a fluorophore with an excitation peak at 695 nm and an emission peak at 720 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 700 and Cy5.5.
- What laser excites CF700?
- CF700 is optimally excited by the 628-640 laser line. Its excitation maximum is 695 nm.
- What filter should I use to detect CF700?
- A 710/50 filter captures the peak of the CF700 emission near 720 nm with minimal spillover.
- How bright is CF700?
- CF700 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF700?
- FluoroFinder tracks 40 commercially available CF700 conjugates, searchable by target, clone, host and application.