CF550R
CF®550R (Ex-Max 551 nm/Em-Max 577 nm)
CF®550R from Biotium is a fluorophore with an excitation peak at 551 nm and an emission peak at 577 nm. It can be useful in both fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 551 nm
- Emission maximum
- 577 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 100000
- Molecular weight
- 686
CF550R has an excitation maximum at 551 nm and an emission maximum at 577 nm, a Stokes shift of 26 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is CF550R?
- CF®550R from Biotium is a fluorophore with an excitation peak at 551 nm and an emission peak at 577 nm. It can be useful in both fluorescence microscopy and flow cytometry.
- What laser excites CF550R?
- CF550R is optimally excited by the 561 laser line. Its excitation maximum is 551 nm.
- What filter should I use to detect CF550R?
- A 585/42 filter captures the peak of the CF550R emission near 577 nm with minimal spillover.
- How bright is CF550R?
- CF550R has a relative brightness of 2 out of 5.