CF503R
CF®503R (Ex-Max 503 nm/Em-Max 532 nm)
CF®503R from Biotium is a fluorophore with an excitation peak at 503 nm and an emission peak at 532 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 503 nm
- Emission maximum
- 532 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 90000
- Molecular weight
- 1100
- Commercial antibodies
- 6 from 1 supplier
CF503R has an excitation maximum at 503 nm and an emission maximum at 532 nm, a Stokes shift of 29 nm. Its extinction coefficient is 90000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CF503R?
- CF®503R from Biotium is a fluorophore with an excitation peak at 503 nm and an emission peak at 532 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry.
- What laser excites CF503R?
- CF503R is optimally excited by the 488 laser line. Its excitation maximum is 503 nm.
- What filter should I use to detect CF503R?
- A 525/50 filter captures the peak of the CF503R emission near 532 nm with minimal spillover.
- How bright is CF503R?
- CF503R has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF503R?
- FluoroFinder tracks 6 commercially available CF503R conjugates, searchable by target, clone, host and application.