Brilliant Ultraviolet 395
Brilliant™ UV 395 (BUV395)
Brilliant™ UltraViolet 395 (BUV395) is a UV-emitting base polymer dye that can be excited by the 355 nm UV laser and collected using a 379/28 bandpass filter. BUV395 has an excitation peak at 348 nm and an emission peak at 395. BUV395's fairly unique excitation and emission spectrum causes relatively little spillover into other common detectors and conflicts with very few other commonly used fluorophores. An alternative to BUV395 for flow cytometry is StarBright UltraViolet 400. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. These dyes were optimized for use with multicolor flow cytometry. BUV395 forms the backbone of the family, as all other members are tandems that use it as the acceptor, thus making them excitable by the same 355 nm UV laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 348 nm
- Emission maximum
- 395 nm
- Optimal laser line
- 355
- Optimal filter
- 379/28
- Relative brightness
- 2 of 5
- Commercial antibodies
- 1.1k from 3 suppliers
Brilliant Ultraviolet 395 has an excitation maximum at 348 nm and an emission maximum at 395 nm, a Stokes shift of 47 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Brilliant Ultraviolet 395?
- Brilliant™ UltraViolet 395 (BUV395) is a UV-emitting base polymer dye that can be excited by the 355 nm UV laser and collected using a 379/28 bandpass filter. BUV395 has an excitation peak at 348 nm and an emission peak at 395. BUV395's fairly unique excitation and emission spectrum causes relatively little spillover into other common detectors and conflicts with very few other commonly used fluorophores. An alternative to BUV395 for flow cytometry is StarBright UltraViolet 400. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. These dyes were optimized for use with multicolor flow cytometry. BUV395 forms the backbone of the family, as all other members are tandems that use it as the acceptor, thus making them excitable by the same 355 nm UV laser.
- What laser excites Brilliant Ultraviolet 395?
- Brilliant Ultraviolet 395 is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.
- What filter should I use to detect Brilliant Ultraviolet 395?
- A 379/28 filter captures the peak of the Brilliant Ultraviolet 395 emission near 395 nm with minimal spillover.
- How bright is Brilliant Ultraviolet 395?
- Brilliant Ultraviolet 395 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Brilliant Ultraviolet 395?
- FluoroFinder tracks 1077 commercially available Brilliant Ultraviolet 395 conjugates, searchable by target, clone, host and application.