GelGreen
GelGreen (Ex-Max 507 nm/Em-Max 528 nm)
GelGreen from Biotium is a fluorogenic DNA staining dye similar to SYBR Green I for in-gel detection and quantitation of DNA. It has an excitation peak at 507 nm and an emission peak at 528 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 507 nm
- Emission maximum
- 528 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
GelGreen has an excitation maximum at 507 nm and an emission maximum at 528 nm, a Stokes shift of 21 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is GelGreen?
- GelGreen from Biotium is a fluorogenic DNA staining dye similar to SYBR Green I for in-gel detection and quantitation of DNA. It has an excitation peak at 507 nm and an emission peak at 528 nm.
- What laser excites GelGreen?
- GelGreen is optimally excited by the 488 laser line. Its excitation maximum is 507 nm.
- What filter should I use to detect GelGreen?
- A 525/50 filter captures the peak of the GelGreen emission near 528 nm with minimal spillover.