GelRed
GelRed from Biotium is a fluorogenic DNA staining dye similar to Ethidium Bromide for in-gel detection and quantitation of DNA. It has an excitation peak at 279 nm and an emission peak at 593 nm and can be visualized using a UV transillumination device.
Excitation & emission spectra
Key facts
- Excitation maximum
- 279 nm
- Emission maximum
- 593 nm
- Optimal laser line
- 355
- Optimal filter
- 585/42
GelRed has an excitation maximum at 279 nm and an emission maximum at 593 nm, a Stokes shift of 314 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is GelRed?
- GelRed from Biotium is a fluorogenic DNA staining dye similar to Ethidium Bromide for in-gel detection and quantitation of DNA. It has an excitation peak at 279 nm and an emission peak at 593 nm and can be visualized using a UV transillumination device.
- What laser excites GelRed?
- GelRed is optimally excited by the 355 laser line. Its excitation maximum is 279 nm.
- What filter should I use to detect GelRed?
- A 585/42 filter captures the peak of the GelRed emission near 593 nm with minimal spillover.