LysoView 405
LysoView™ 405 (Ex-Max 400 nm/Em-Max 464 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 405 has an excitation peak at 400 nm and an emission peak at 464 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 400 nm
- Emission maximum
- 464 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Commercial antibodies
- 2 from 1 supplier
LysoView 405 has an excitation maximum at 400 nm and an emission maximum at 464 nm, a Stokes shift of 64 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is LysoView 405?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 405 has an excitation peak at 400 nm and an emission peak at 464 nm.
- What laser excites LysoView 405?
- LysoView 405 is optimally excited by the 405 laser line. Its excitation maximum is 400 nm.
- What filter should I use to detect LysoView 405?
- A 450/50 filter captures the peak of the LysoView 405 emission near 464 nm with minimal spillover.
- How many antibodies are available conjugated to LysoView 405?
- FluoroFinder tracks 2 commercially available LysoView 405 conjugates, searchable by target, clone, host and application.