Live-or-Dye 787/808
Live-or-Dye™ 787/808 (Ex-Max 783 nm/Em-Max 807 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 787/808 has an excitation peak at 783 nm and an emission peak at 807 nm. In flow cytometry, this probe requires either a 785 or 808 nm laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 783 nm
- Emission maximum
- 807 nm
- Optimal laser line
- 785
- Optimal filter
- 780/60
- Commercial antibodies
- 4 from 2 suppliers
Live-or-Dye 787/808 has an excitation maximum at 783 nm and an emission maximum at 807 nm, a Stokes shift of 24 nm. It is optimally excited by the 785 laser line.
Frequently asked questions
- What is Live-or-Dye 787/808?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 787/808 has an excitation peak at 783 nm and an emission peak at 807 nm. In flow cytometry, this probe requires either a 785 or 808 nm laser.
- What laser excites Live-or-Dye 787/808?
- Live-or-Dye 787/808 is optimally excited by the 785 laser line. Its excitation maximum is 783 nm.
- What filter should I use to detect Live-or-Dye 787/808?
- A 780/60 filter captures the peak of the Live-or-Dye 787/808 emission near 807 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 787/808?
- FluoroFinder tracks 4 commercially available Live-or-Dye 787/808 conjugates, searchable by target, clone, host and application.