Live-or-Dye 665/685
Live-or-Dye™ 665/685 (Ex-Max 667 nm/Em-Max 685 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 665/685 has an excitation peak at 665 nm and an emission peak at 685 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 667 nm
- Emission maximum
- 685 nm
- Optimal laser line
- 628-640
- Optimal filter
- N/A (Spectral)
- Commercial antibodies
- 4 from 2 suppliers
Live-or-Dye 665/685 has an excitation maximum at 667 nm and an emission maximum at 685 nm, a Stokes shift of 18 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Live-or-Dye 665/685?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 665/685 has an excitation peak at 665 nm and an emission peak at 685 nm.
- What laser excites Live-or-Dye 665/685?
- Live-or-Dye 665/685 is optimally excited by the 628-640 laser line. Its excitation maximum is 667 nm.
- What filter should I use to detect Live-or-Dye 665/685?
- A N/A (Spectral) filter captures the peak of the Live-or-Dye 665/685 emission near 685 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 665/685?
- FluoroFinder tracks 4 commercially available Live-or-Dye 665/685 conjugates, searchable by target, clone, host and application.