Live-or-Dye 510/550
Live-or-Dye™ 510/550 (Ex-Max 516 nm/Em-Max 548 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 510/550 has an excitation peak at 510 nm and an emission peak at 550 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 548 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Commercial conjugates
- 2 from 1 supplier
Live-or-Dye 510/550 has an excitation maximum at 516 nm and an emission maximum at 548 nm, a Stokes shift of 32 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Live-or-Dye 510/550?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 510/550 has an excitation peak at 510 nm and an emission peak at 550 nm.
- What laser excites Live-or-Dye 510/550?
- Live-or-Dye 510/550 is optimally excited by the 488 laser line. Its excitation maximum is 516 nm.
- What filter should I use to detect Live-or-Dye 510/550?
- A 525/50 filter captures the peak of the Live-or-Dye 510/550 emission near 548 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 510/550?
- FluoroFinder tracks 2 commercially available Live-or-Dye 510/550 conjugates, searchable by target, clone, host and application.