Resorufin
Resorufin (Ex-Max 571 nm/Em-Max 584 nm)
Resorufin is excited by the 561 laser and emission captured by the 585/42 filter. It can be used with the following analysis platforms: Substrate, ELISA, Immunohistochemistry, Western Blot.
Excitation & emission spectra
Key facts
- Excitation maximum
- 571 nm
- Emission maximum
- 584 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
Resorufin has an excitation maximum at 571 nm and an emission maximum at 584 nm, a Stokes shift of 13 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Resorufin?
- Resorufin is excited by the 561 laser and emission captured by the 585/42 filter. It can be used with the following analysis platforms: Substrate, ELISA, Immunohistochemistry, Western Blot.
- What laser excites Resorufin?
- Resorufin is optimally excited by the 561 laser line. Its excitation maximum is 571 nm.
- What filter should I use to detect Resorufin?
- A 585/42 filter captures the peak of the Resorufin emission near 584 nm with minimal spillover.