NBD
NBD (Ex-Max 467 nm/Em-Max 538 nm)
NBD (nitrobenzodiazole) is a hydrophobic fluorophore used to label and track cellular lipids. It has excitation/ emission peaks at 467 nm and 538 nm, respectively.
Excitation & emission spectra
Key facts
- Excitation maximum
- 467 nm
- Emission maximum
- 538 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Molecular weight
- 223
NBD has an excitation maximum at 467 nm and an emission maximum at 538 nm, a Stokes shift of 71 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NBD?
- NBD (nitrobenzodiazole) is a hydrophobic fluorophore used to label and track cellular lipids. It has excitation/ emission peaks at 467 nm and 538 nm, respectively.
- What laser excites NBD?
- NBD is optimally excited by the 488 laser line. Its excitation maximum is 467 nm.
- What filter should I use to detect NBD?
- A 525/50 filter captures the peak of the NBD emission near 538 nm with minimal spillover.