NucSpot Live 650
NucSpot Live 650 (Ex-Max 655 nm/Em-Max 681 nm)
Biotium's NucSpot Live 650 is a cell membrane-permeant dye that can passively enter live cells to assess DNA content analysis or counterstain nuclei in flow cytometry and fluorescence microscopy applications. It has an excitation peak at 655 nm and an emission peak at 681 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 655 nm
- Emission maximum
- 681 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Commercial antibodies
- 2 from 1 supplier
NucSpot Live 650 has an excitation maximum at 655 nm and an emission maximum at 681 nm, a Stokes shift of 26 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is NucSpot Live 650?
- Biotium's NucSpot Live 650 is a cell membrane-permeant dye that can passively enter live cells to assess DNA content analysis or counterstain nuclei in flow cytometry and fluorescence microscopy applications. It has an excitation peak at 655 nm and an emission peak at 681 nm.
- What laser excites NucSpot Live 650?
- NucSpot Live 650 is optimally excited by the 628-640 laser line. Its excitation maximum is 655 nm.
- What filter should I use to detect NucSpot Live 650?
- A 695/40 filter captures the peak of the NucSpot Live 650 emission near 681 nm with minimal spillover.
- How many antibodies are available conjugated to NucSpot Live 650?
- FluoroFinder tracks 2 commercially available NucSpot Live 650 conjugates, searchable by target, clone, host and application.