iFluor 350
iFluor® 350 (Ex-Max 345 nm/Em-Max 450 nm)
iFluor® 350 from AAT Bioquest is a fluorophore with an excitation peak at 345 nm and an emission peak at 450nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 350 and AMCA.
Excitation & emission spectra
Key facts
- Excitation maximum
- 345 nm
- Emission maximum
- 450 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 20000
- Quantum yield
- 0.95
- Molecular weight
- 749.85
iFluor 350 has an excitation maximum at 345 nm and an emission maximum at 450 nm, a Stokes shift of 105 nm. Its extinction coefficient is 20000 M⁻¹cm⁻¹. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is iFluor 350?
- iFluor® 350 from AAT Bioquest is a fluorophore with an excitation peak at 345 nm and an emission peak at 450nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 350 and AMCA.
- What laser excites iFluor 350?
- iFluor 350 is optimally excited by the 355 laser line. Its excitation maximum is 345 nm.
- What filter should I use to detect iFluor 350?
- A 450/50 filter captures the peak of the iFluor 350 emission near 450 nm with minimal spillover.
- How bright is iFluor 350?
- iFluor 350 has a relative brightness of 1 out of 5 (quantum yield 0.95).