DiFMU pH3.0
DiFMU pH3.0 (Ex-Max 318 nm/Em-Max 448 nm)
DiFMU, also known as (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), can be used as a reference standard for ether, ester and phosphate substrates derived from the methylcoumarin dye.
Excitation & emission spectra
Key facts
- Excitation maximum
- 318 nm
- Emission maximum
- 448 nm
- Optimal laser line
- 325
- Optimal filter
- 450/50
- Molecular weight
- 212.15
DiFMU pH3.0 has an excitation maximum at 318 nm and an emission maximum at 448 nm, a Stokes shift of 130 nm. It is optimally excited by the 325 laser line.
Frequently asked questions
- What is DiFMU pH3.0?
- DiFMU, also known as (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), can be used as a reference standard for ether, ester and phosphate substrates derived from the methylcoumarin dye.
- What laser excites DiFMU pH3.0?
- DiFMU pH3.0 is optimally excited by the 325 laser line. Its excitation maximum is 318 nm.
- What filter should I use to detect DiFMU pH3.0?
- A 450/50 filter captures the peak of the DiFMU pH3.0 emission near 448 nm with minimal spillover.