DiFMU pH9.0
DiFMU pH9.0 (Ex-Max 355 nm/Em-Max 448 nm)
DiFMU, also known as (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), can be used as a reference standard for ether, ester and phosphate substrates derived from the methylcoumarin dye.
Excitation & emission spectra
Key facts
- Excitation maximum
- 355 nm
- Emission maximum
- 448 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Molecular weight
- 212.15
DiFMU pH9.0 has an excitation maximum at 355 nm and an emission maximum at 448 nm, a Stokes shift of 93 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is DiFMU pH9.0?
- DiFMU, also known as (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), can be used as a reference standard for ether, ester and phosphate substrates derived from the methylcoumarin dye.
- What laser excites DiFMU pH9.0?
- DiFMU pH9.0 is optimally excited by the 355 laser line. Its excitation maximum is 355 nm.
- What filter should I use to detect DiFMU pH9.0?
- A 450/50 filter captures the peak of the DiFMU pH9.0 emission near 448 nm with minimal spillover.