Rhod-5N (Ca2+ bound)
Rhod-5N (Ca2+ bound) (Ex-Max 555 nm/Em-Max 578 nm)
Rhod-5N is a calcium indicator used in Fluorescence Microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 555 nm
- Emission maximum
- 578 nm
- Optimal laser line
- 532-561
- Optimal filter
- 585/42
Rhod-5N (Ca2+ bound) has an excitation maximum at 555 nm and an emission maximum at 578 nm, a Stokes shift of 23 nm. It is optimally excited by the 532-561 laser line.
Frequently asked questions
- What is Rhod-5N (Ca2+ bound)?
- Rhod-5N is a calcium indicator used in Fluorescence Microscopy applications.
- What laser excites Rhod-5N (Ca2+ bound)?
- Rhod-5N (Ca2+ bound) is optimally excited by the 532-561 laser line. Its excitation maximum is 555 nm.
- What filter should I use to detect Rhod-5N (Ca2+ bound)?
- A 585/42 filter captures the peak of the Rhod-5N (Ca2+ bound) emission near 578 nm with minimal spillover.