BODIPY 581/591
BODIPY 581/591 (Ex-Max 586 nm/Em-Max 596 nm)
BODIPY 581/591 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 581 nm and an emission peak at 591 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
Excitation & emission spectra
Key facts
- Excitation maximum
- 586 nm
- Emission maximum
- 596 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Molecular weight
- 504.4255
BODIPY 581/591 has an excitation maximum at 586 nm and an emission maximum at 596 nm, a Stokes shift of 10 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is BODIPY 581/591?
- BODIPY 581/591 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 581 nm and an emission peak at 591 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
- What laser excites BODIPY 581/591?
- BODIPY 581/591 is optimally excited by the 561 laser line. Its excitation maximum is 586 nm.
- What filter should I use to detect BODIPY 581/591?
- A 585/42 filter captures the peak of the BODIPY 581/591 emission near 596 nm with minimal spillover.