APC-Fire 810
APC-Fire™ 810 (Ex-Max 650 nm/Em-Max 807 nm)
APC-Fire™ 810 from BioLegend has an excitation peak at 650nm and an emission peak at 807 nm and is spectrally similar to APC-CF®790. APC-Fire™ 810 is most commonly used in flow cytometry. On a 5-laser Cytek® Aurora, it has very limited spectral spillover into any other channel, so it can be assigned to any antigen with moderate to abundant expression and used in combination with any other fluorophore. This dye is very useful for detecting variably expressed markers, like markers of activation, since it emits so far outside of the autofluorescence range.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 807 nm
- Optimal laser line
- 628-640
- Optimal filter
- 780/60
- Relative brightness
- 3 of 5
- Commercial antibodies
- 367 from 2 suppliers
APC-Fire 810 has an excitation maximum at 650 nm and an emission maximum at 807 nm, a Stokes shift of 157 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is APC-Fire 810?
- APC-Fire™ 810 from BioLegend has an excitation peak at 650nm and an emission peak at 807 nm and is spectrally similar to APC-CF®790. APC-Fire™ 810 is most commonly used in flow cytometry. On a 5-laser Cytek® Aurora, it has very limited spectral spillover into any other channel, so it can be assigned to any antigen with moderate to abundant expression and used in combination with any other fluorophore. This dye is very useful for detecting variably expressed markers, like markers of activation, since it emits so far outside of the autofluorescence range.
- What laser excites APC-Fire 810?
- APC-Fire 810 is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
- What filter should I use to detect APC-Fire 810?
- A 780/60 filter captures the peak of the APC-Fire 810 emission near 807 nm with minimal spillover.
- How bright is APC-Fire 810?
- APC-Fire 810 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to APC-Fire 810?
- FluoroFinder tracks 367 commercially available APC-Fire 810 conjugates, searchable by target, clone, host and application.