PE-Fire 640
PE-Fire™ 640 (Ex-Max 566 nm/Em-Max 639 nm)
PE-Fire™ 640 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 640. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 640, which emitts light that can be captured with a 630/69 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 639 nm. In spectral cytometry, this dye can be accurately unmixed from other dyes with highly overlapping spectral profiles, such as PE-Dazzle® 594 and PE-Cy5. PE-Fire™ 640 is recommended for labeling antigens that: (1) are expressed on a small subset (2) will be excluded from final analysis, and (3) require a bright signal but are not co-expressed with an antigen marked by a dye with overlapping spectra.
Excitation & emission spectra
Key facts
- Excitation maximum
- 566 nm
- Emission maximum
- 639 nm
- Optimal laser line
- 488-561
- Optimal filter
- 630/69
- Relative brightness
- 4 of 5
- Commercial antibodies
- 332 from 2 suppliers
PE-Fire 640 has an excitation maximum at 566 nm and an emission maximum at 639 nm, a Stokes shift of 73 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Fire 640?
- PE-Fire™ 640 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 640. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 640, which emitts light that can be captured with a 630/69 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 639 nm. In spectral cytometry, this dye can be accurately unmixed from other dyes with highly overlapping spectral profiles, such as PE-Dazzle® 594 and PE-Cy5. PE-Fire™ 640 is recommended for labeling antigens that: (1) are expressed on a small subset (2) will be excluded from final analysis, and (3) require a bright signal but are not co-expressed with an antigen marked by a dye with overlapping spectra.
- What laser excites PE-Fire 640?
- PE-Fire 640 is optimally excited by the 488-561 laser line. Its excitation maximum is 566 nm.
- What filter should I use to detect PE-Fire 640?
- A 630/69 filter captures the peak of the PE-Fire 640 emission near 639 nm with minimal spillover.
- How bright is PE-Fire 640?
- PE-Fire 640 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to PE-Fire 640?
- FluoroFinder tracks 332 commercially available PE-Fire 640 conjugates, searchable by target, clone, host and application.