NovaFluor Blue 660 / 40S
NovaFluor Blue 660 / 40S (Ex-Max 509 nm/Em-Max 665 nm)
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 660/ 40S is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 496 nm and an emission peak at 665nm. NovaFluor Blue 660/ 40S is smaller and dimmer than NovaFluor Blue 660/ 120S.
Excitation & emission spectra
Key facts
- Excitation maximum
- 509 nm
- Emission maximum
- 665 nm
- Optimal laser line
- 488
- Optimal filter
- 660/20
- Relative brightness
- 1 of 5
- Commercial antibodies
- 32 from 1 supplier
NovaFluor Blue 660 / 40S has an excitation maximum at 509 nm and an emission maximum at 665 nm, a Stokes shift of 156 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NovaFluor Blue 660 / 40S?
- The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 660/ 40S is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 496 nm and an emission peak at 665nm. NovaFluor Blue 660/ 40S is smaller and dimmer than NovaFluor Blue 660/ 120S.
- What laser excites NovaFluor Blue 660 / 40S?
- NovaFluor Blue 660 / 40S is optimally excited by the 488 laser line. Its excitation maximum is 509 nm.
- What filter should I use to detect NovaFluor Blue 660 / 40S?
- A 660/20 filter captures the peak of the NovaFluor Blue 660 / 40S emission near 665 nm with minimal spillover.
- How bright is NovaFluor Blue 660 / 40S?
- NovaFluor Blue 660 / 40S has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to NovaFluor Blue 660 / 40S?
- FluoroFinder tracks 32 commercially available NovaFluor Blue 660 / 40S conjugates, searchable by target, clone, host and application.