Chromeo 494
Chromeo 494 (Ex-Max 494 nm/Em-Max 628 nm)
Chromeo® 494 is a unique red fluorescent label that is excitable at a wavelength of 488 nm by Argon Laser, LED, Xenon-Arc or Tungsten-Halogen lamps. It has a very large Stokes shift of 134 nm, making it an ideal partner for multiplexing in immunofluorescence experiments, especially in combination with Chromeo 488. While both dyes are readily excited at 488 nm, the fluorescent emission of Chromeo 488 at 517 nm is easily distinguished from the emission of Chromeo 494 at 628 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 628 nm
- Optimal laser line
- 488
- Optimal filter
- 630/69
- Relative brightness
- 2 of 5
- Extinction coefficient
- 55000
Chromeo 494 has an excitation maximum at 494 nm and an emission maximum at 628 nm, a Stokes shift of 134 nm. Its extinction coefficient is 55000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Chromeo 494?
- Chromeo® 494 is a unique red fluorescent label that is excitable at a wavelength of 488 nm by Argon Laser, LED, Xenon-Arc or Tungsten-Halogen lamps. It has a very large Stokes shift of 134 nm, making it an ideal partner for multiplexing in immunofluorescence experiments, especially in combination with Chromeo 488. While both dyes are readily excited at 488 nm, the fluorescent emission of Chromeo 488 at 517 nm is easily distinguished from the emission of Chromeo 494 at 628 nm.
- What laser excites Chromeo 494?
- Chromeo 494 is optimally excited by the 488 laser line. Its excitation maximum is 494 nm.
- What filter should I use to detect Chromeo 494?
- A 630/69 filter captures the peak of the Chromeo 494 emission near 628 nm with minimal spillover.
- How bright is Chromeo 494?
- Chromeo 494 has a relative brightness of 2 out of 5.