KIRAVIA Blue 520
KIRAVIA Blue 520 (Ex-Max 497 nm/Em-Max 520 nm)
KIRAVIA Blue 520® (KB520) is a polymeric fluorophore from Sony Biotechnology that has an excitation peak at 497 nm and an emission peak at 520 nm, and is considered an improved alternative to FITC.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 520 nm
- Optimal laser line
- 488
- Optimal filter
- 520/30
- Relative brightness
- 2 of 5
- Commercial antibodies
- 279 from 2 suppliers
KIRAVIA Blue 520 has an excitation maximum at 497 nm and an emission maximum at 520 nm, a Stokes shift of 23 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is KIRAVIA Blue 520?
- KIRAVIA Blue 520® (KB520) is a polymeric fluorophore from Sony Biotechnology that has an excitation peak at 497 nm and an emission peak at 520 nm, and is considered an improved alternative to FITC.
- What laser excites KIRAVIA Blue 520?
- KIRAVIA Blue 520 is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect KIRAVIA Blue 520?
- A 520/30 filter captures the peak of the KIRAVIA Blue 520 emission near 520 nm with minimal spillover.
- How bright is KIRAVIA Blue 520?
- KIRAVIA Blue 520 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to KIRAVIA Blue 520?
- FluoroFinder tracks 279 commercially available KIRAVIA Blue 520 conjugates, searchable by target, clone, host and application.