IRDye 680LT
IRDye® 680LT (Ex-Max 676 nm/Em-Max 693 nm)
IRDye® 680LT from Licor has an excitation peak at 676 nm and an emission peak at 693 nm. It can be useful in flow cytometry, fluorescence microscopy, fluorescent western blot detection and in vivo imaging. Spectrally it is similar to Alexa Fluor™ 680.
Excitation & emission spectra
Key facts
- Excitation maximum
- 676 nm
- Emission maximum
- 693 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 2 of 5
- Commercial antibodies
- 23 from 1 supplier
IRDye 680LT has an excitation maximum at 676 nm and an emission maximum at 693 nm, a Stokes shift of 17 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is IRDye 680LT?
- IRDye® 680LT from Licor has an excitation peak at 676 nm and an emission peak at 693 nm. It can be useful in flow cytometry, fluorescence microscopy, fluorescent western blot detection and in vivo imaging. Spectrally it is similar to Alexa Fluor™ 680.
- What laser excites IRDye 680LT?
- IRDye 680LT is optimally excited by the 628-640 laser line. Its excitation maximum is 676 nm.
- What filter should I use to detect IRDye 680LT?
- A 695/40 filter captures the peak of the IRDye 680LT emission near 693 nm with minimal spillover.
- How bright is IRDye 680LT?
- IRDye 680LT has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to IRDye 680LT?
- FluoroFinder tracks 23 commercially available IRDye 680LT conjugates, searchable by target, clone, host and application.