IRDye 650
IRDye® 650 (Ex-Max 651 nm/Em-Max 668 nm)
IRDye® 650 from Licor has an excitation peak at 651 nm and an emission peak at 668 nm. It can be useful in flow cytometry, fluorescence microscopy, fluorescent western blot detection and in vivo imaging. Spectrally it is similar to Alexa Fluor™ 647.
Excitation & emission spectra
Key facts
- Excitation maximum
- 651 nm
- Emission maximum
- 668 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 4 of 5
- Commercial antibodies
- 2 from 1 supplier
IRDye 650 has an excitation maximum at 651 nm and an emission maximum at 668 nm, a Stokes shift of 17 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is IRDye 650?
- IRDye® 650 from Licor has an excitation peak at 651 nm and an emission peak at 668 nm. It can be useful in flow cytometry, fluorescence microscopy, fluorescent western blot detection and in vivo imaging. Spectrally it is similar to Alexa Fluor™ 647.
- What laser excites IRDye 650?
- IRDye 650 is optimally excited by the 628-640 laser line. Its excitation maximum is 651 nm.
- What filter should I use to detect IRDye 650?
- A 660/20 filter captures the peak of the IRDye 650 emission near 668 nm with minimal spillover.
- How bright is IRDye 650?
- IRDye 650 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to IRDye 650?
- FluoroFinder tracks 2 commercially available IRDye 650 conjugates, searchable by target, clone, host and application.