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Seta-R-PE-775

Seta-R-PE-775 (Ex-Max 488 nm/Em-Max 774 nm)

Seta-R-PE-775 from SETA BioMedicals is a tandem fluorophore with an excitation peak at 488 nm and an emission peak at 774 nm. Tandem dyes made with Seta acceptor fluorophores are available as free dye for your own conjugation. Spectrally, this fluorophore is similar to PE-Cy7.

Excitation & emission spectra

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Key facts

Excitation maximum
488 nm
Emission maximum
774 nm
Optimal laser line
488-561
Optimal filter
780/60
Relative brightness
4 of 5
Extinction coefficient
1100000

Seta-R-PE-775 has an excitation maximum at 488 nm and an emission maximum at 774 nm, a Stokes shift of 286 nm. Its extinction coefficient is 1100000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.

Frequently asked questions

What is Seta-R-PE-775?
Seta-R-PE-775 from SETA BioMedicals is a tandem fluorophore with an excitation peak at 488 nm and an emission peak at 774 nm. Tandem dyes made with Seta acceptor fluorophores are available as free dye for your own conjugation. Spectrally, this fluorophore is similar to PE-Cy7.
What laser excites Seta-R-PE-775?
Seta-R-PE-775 is optimally excited by the 488-561 laser line. Its excitation maximum is 488 nm.
What filter should I use to detect Seta-R-PE-775?
A 780/60 filter captures the peak of the Seta-R-PE-775 emission near 774 nm with minimal spillover.
How bright is Seta-R-PE-775?
Seta-R-PE-775 has a relative brightness of 4 out of 5.