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Rhodamine

Rhodamine (Ex-Max 558 nm/Em-Max 575 nm)

Rhodamine is a generic fluorophore that has an excitation peak at 558 nm and an emission peak at 575 nm. Spectrally, it's similar to Alexa Fluor™ 546.

Excitation & emission spectra

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Key facts

Excitation maximum
558 nm
Emission maximum
575 nm
Optimal laser line
561
Optimal filter
585/42
Molecular weight
1250
Commercial antibodies
164 from 16 suppliers

Rhodamine has an excitation maximum at 558 nm and an emission maximum at 575 nm, a Stokes shift of 17 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What is Rhodamine?
Rhodamine is a generic fluorophore that has an excitation peak at 558 nm and an emission peak at 575 nm. Spectrally, it's similar to Alexa Fluor™ 546.
What laser excites Rhodamine?
Rhodamine is optimally excited by the 561 laser line. Its excitation maximum is 558 nm.
What filter should I use to detect Rhodamine?
A 585/42 filter captures the peak of the Rhodamine emission near 575 nm with minimal spillover.
How many antibodies are available conjugated to Rhodamine?
FluoroFinder tracks 164 commercially available Rhodamine conjugates, searchable by target, clone, host and application.