Riboflavin
Riboflavin (Ex-Max 360 nm/Em-Max 530 nm)
Riboflavin (Vitamin B2) is a naturally occurring compound that plays a role in many vital chemical processes in living organisms. In our bodies, flavonoids have anti-oxidant activity by absorbing short, potentially harmful wavelengths of light. Structurally, flavonoids are very similar to families of synthetic organic fluorophores due to the three fused benzene rings called an anthracene. Hence, Riboflavin can contribute to the endogenous autofluorescence of cells and tissues.
Excitation & emission spectra
Key facts
- Excitation maximum
- 360 nm
- Emission maximum
- 530 nm
- Optimal laser line
- 355
- Optimal filter
- 525/50
- Relative brightness
- 3 of 5
Riboflavin has an excitation maximum at 360 nm and an emission maximum at 530 nm, a Stokes shift of 170 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Riboflavin?
- Riboflavin (Vitamin B2) is a naturally occurring compound that plays a role in many vital chemical processes in living organisms. In our bodies, flavonoids have anti-oxidant activity by absorbing short, potentially harmful wavelengths of light. Structurally, flavonoids are very similar to families of synthetic organic fluorophores due to the three fused benzene rings called an anthracene. Hence, Riboflavin can contribute to the endogenous autofluorescence of cells and tissues.
- What laser excites Riboflavin?
- Riboflavin is optimally excited by the 355 laser line. Its excitation maximum is 360 nm.
- What filter should I use to detect Riboflavin?
- A 525/50 filter captures the peak of the Riboflavin emission near 530 nm with minimal spillover.
- How bright is Riboflavin?
- Riboflavin has a relative brightness of 3 out of 5.