Fluorescein
Fluorescein (Ex-Max 494 nm/Em-Max 512 nm)
Fluorescein is an organic fluorophore first synthesized by Aldolf Bayer in 1871. FITC, an amine-reactive derivative, is also a commonly used name for this dye. It has an excitation peak at 494 nm and an emission peak at 512 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 512 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 70000
- Commercial antibodies
- 10.5k from 8 suppliers
Fluorescein has an excitation maximum at 494 nm and an emission maximum at 512 nm, a Stokes shift of 18 nm. Its extinction coefficient is 70000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Fluorescein?
- Fluorescein is an organic fluorophore first synthesized by Aldolf Bayer in 1871. FITC, an amine-reactive derivative, is also a commonly used name for this dye. It has an excitation peak at 494 nm and an emission peak at 512 nm.
- What laser excites Fluorescein?
- Fluorescein is optimally excited by the 488 laser line. Its excitation maximum is 494 nm.
- What filter should I use to detect Fluorescein?
- A 525/50 filter captures the peak of the Fluorescein emission near 512 nm with minimal spillover.
- How bright is Fluorescein?
- Fluorescein has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Fluorescein?
- FluoroFinder tracks 10543 commercially available Fluorescein conjugates, searchable by target, clone, host and application.