Rhodamine B
Rhodamine B (Ex-Max 545 nm/Em-Max 566 nm)
Rhodamine B is a generic fluorophore that has an excitation peak at 545 nm and an emission peak at 566 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 545 nm
- Emission maximum
- 566 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Molecular weight
- 479.01
Rhodamine B has an excitation maximum at 545 nm and an emission maximum at 566 nm, a Stokes shift of 21 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Rhodamine B?
- Rhodamine B is a generic fluorophore that has an excitation peak at 545 nm and an emission peak at 566 nm.
- What laser excites Rhodamine B?
- Rhodamine B is optimally excited by the 532 laser line. Its excitation maximum is 545 nm.
- What filter should I use to detect Rhodamine B?
- A 585/42 filter captures the peak of the Rhodamine B emission near 566 nm with minimal spillover.
- How bright is Rhodamine B?
- Rhodamine B has a relative brightness of 2 out of 5.