StarBright Blue 520
StarBright Blue 520 (Ex-Max 475 nm/Em-Max 582 nm)
StarBright Blue 520 (SBB520) is a fluorescent polymer nanoparticle with an excitation peak at 440 nm and an emission peak at 513 nm. The StarBright family fluorophores from Bio-Rad offers an alternative to the Horizon Brilliant™ polymer series from BD including Brilliant™ Violet and Brilliant™ UV.
Excitation & emission spectra
Key facts
- Excitation maximum
- 440 nm
- Emission maximum
- 513 nm
- Optimal laser line
- 405
- Optimal filter
- 525/50
- Relative brightness
- 3 of 5
- Commercial antibodies
- 4 from 1 supplier
StarBright Blue 520 has an excitation maximum at 440 nm and an emission maximum at 513 nm, a Stokes shift of 73 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is StarBright Blue 520?
- StarBright Blue 520 (SBB520) is a fluorescent polymer nanoparticle with an excitation peak at 440 nm and an emission peak at 513 nm. The StarBright family fluorophores from Bio-Rad offers an alternative to the Horizon Brilliant™ polymer series from BD including Brilliant™ Violet and Brilliant™ UV.
- What laser excites StarBright Blue 520?
- StarBright Blue 520 is optimally excited by the 405 laser line. Its excitation maximum is 440 nm.
- What filter should I use to detect StarBright Blue 520?
- A 525/50 filter captures the peak of the StarBright Blue 520 emission near 513 nm with minimal spillover.
- How bright is StarBright Blue 520?
- StarBright Blue 520 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to StarBright Blue 520?
- FluoroFinder tracks 4 commercially available StarBright Blue 520 conjugates, searchable by target, clone, host and application.