Ghost Dye Violet 540
Ghost Dye™ Violet 540 (Ex-Max 398 nm/Em-Max 541 nm)
Ghost Dye™ Violet 540 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 398 nm and an emission peak at 541 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 398 nm
- Emission maximum
- 541 nm
- Optimal laser line
- 405
- Optimal filter
- 525/50
- Commercial antibodies
- 3 from 2 suppliers
Ghost Dye Violet 540 has an excitation maximum at 398 nm and an emission maximum at 541 nm, a Stokes shift of 143 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Ghost Dye Violet 540?
- Ghost Dye™ Violet 540 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 398 nm and an emission peak at 541 nm.
- What laser excites Ghost Dye Violet 540?
- Ghost Dye Violet 540 is optimally excited by the 405 laser line. Its excitation maximum is 398 nm.
- What filter should I use to detect Ghost Dye Violet 540?
- A 525/50 filter captures the peak of the Ghost Dye Violet 540 emission near 541 nm with minimal spillover.
- How many antibodies are available conjugated to Ghost Dye Violet 540?
- FluoroFinder tracks 3 commercially available Ghost Dye Violet 540 conjugates, searchable by target, clone, host and application.