HiLyte 647
HiLyte™ 647 (Ex-Max 650 nm/Em-Max 675 nm)
HiLyte™ Fluor 647 from AnaSpec has an excitation peak at 650 nm and an emission peak at 675 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 675 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 4 of 5
- Extinction coefficient
- 250000
- Quantum yield
- 0.33
HiLyte 647 has an excitation maximum at 650 nm and an emission maximum at 675 nm, a Stokes shift of 25 nm. Its extinction coefficient is 250000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is HiLyte 647?
- HiLyte™ Fluor 647 from AnaSpec has an excitation peak at 650 nm and an emission peak at 675 nm.
- What laser excites HiLyte 647?
- HiLyte 647 is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
- What filter should I use to detect HiLyte 647?
- A 695/40 filter captures the peak of the HiLyte 647 emission near 675 nm with minimal spillover.
- How bright is HiLyte 647?
- HiLyte 647 has a relative brightness of 4 out of 5 (quantum yield 0.33).