CellTrace Blue
CellTrace Blue (Ex-Max 355 nm/Em-Max 410 nm)
CellTrace® Blue Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. The CellTrace Blue reagent is specifically designed for excitation by a UV laser (at 355 nm or 375 nm) and it can be easily multiplexed with reagents and antibodies excited by other commonly used lasers. This reagent passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 355 nm
- Emission maximum
- 410 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Commercial antibodies
- 1 from 1 supplier
CellTrace Blue has an excitation maximum at 355 nm and an emission maximum at 410 nm, a Stokes shift of 55 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is CellTrace Blue?
- CellTrace® Blue Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. The CellTrace Blue reagent is specifically designed for excitation by a UV laser (at 355 nm or 375 nm) and it can be easily multiplexed with reagents and antibodies excited by other commonly used lasers. This reagent passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm.
- What laser excites CellTrace Blue?
- CellTrace Blue is optimally excited by the 355 laser line. Its excitation maximum is 355 nm.
- What filter should I use to detect CellTrace Blue?
- A 450/50 filter captures the peak of the CellTrace Blue emission near 410 nm with minimal spillover.
- How many antibodies are available conjugated to CellTrace Blue?
- FluoroFinder tracks 1 commercially available CellTrace Blue conjugates, searchable by target, clone, host and application.