iRFP 720
iRFP 720 (Ex-Max 702 nm/Em-Max 720 nm)
iRFP720 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 702 nm and an emission peak at 720 nm. It requires the cofactor biliverdin for fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 702 nm
- Emission maximum
- 720 nm
- Optimal laser line
- 628-640
- Optimal filter
- 710/50
- Extinction coefficient
- 96000
- Quantum yield
- 0.06
- Molecular weight
- 34600
iRFP 720 has an excitation maximum at 702 nm and an emission maximum at 720 nm, a Stokes shift of 18 nm. Its extinction coefficient is 96000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iRFP 720?
- iRFP720 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 702 nm and an emission peak at 720 nm. It requires the cofactor biliverdin for fluorescence.
- What laser excites iRFP 720?
- iRFP 720 is optimally excited by the 628-640 laser line. Its excitation maximum is 702 nm.
- What filter should I use to detect iRFP 720?
- A 710/50 filter captures the peak of the iRFP 720 emission near 720 nm with minimal spillover.