iFluor 680
iFluor® 680 (Ex-Max 684 nm/Em-Max 701 nm)
iFluor® 680 from AAT Bioquest is a fluorophore with an excitation peak at 684 nm and an emission peak at 701 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 680.
Excitation & emission spectra
Key facts
- Excitation maximum
- 684 nm
- Emission maximum
- 701 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 1 of 5
- Extinction coefficient
- 220000
- Quantum yield
- 0.23
- Molecular weight
- 957.17
- Commercial antibodies
- 2 from 2 suppliers
iFluor 680 has an excitation maximum at 684 nm and an emission maximum at 701 nm, a Stokes shift of 17 nm. Its extinction coefficient is 220000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iFluor 680?
- iFluor® 680 from AAT Bioquest is a fluorophore with an excitation peak at 684 nm and an emission peak at 701 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 680.
- What laser excites iFluor 680?
- iFluor 680 is optimally excited by the 628-640 laser line. Its excitation maximum is 684 nm.
- What filter should I use to detect iFluor 680?
- A 695/40 filter captures the peak of the iFluor 680 emission near 701 nm with minimal spillover.
- How bright is iFluor 680?
- iFluor 680 has a relative brightness of 1 out of 5 (quantum yield 0.23).
- How many antibodies are available conjugated to iFluor 680?
- FluoroFinder tracks 2 commercially available iFluor 680 conjugates, searchable by target, clone, host and application.