iFluor 488
iFluor® 488 (Ex-Max 491 nm/Em-Max 516 nm)
iFluor® 488 from AAT Bioquest is a fluorophore with an excitation peak at 491 nm and an emission peak at 516 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 488 and FITC.
Excitation & emission spectra
Key facts
- Excitation maximum
- 491 nm
- Emission maximum
- 516 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 75000
- Quantum yield
- 0.9
- Molecular weight
- 945.07
- Commercial conjugates
- 93 from 7 suppliers
iFluor 488 has an excitation maximum at 491 nm and an emission maximum at 516 nm, a Stokes shift of 25 nm. Its extinction coefficient is 75000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is iFluor 488?
- iFluor® 488 from AAT Bioquest is a fluorophore with an excitation peak at 491 nm and an emission peak at 516 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 488 and FITC.
- What laser excites iFluor 488?
- iFluor 488 is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
- What filter should I use to detect iFluor 488?
- A 525/50 filter captures the peak of the iFluor 488 emission near 516 nm with minimal spillover.
- How bright is iFluor 488?
- iFluor 488 has a relative brightness of 2 out of 5 (quantum yield 0.9).
- How many antibodies are available conjugated to iFluor 488?
- FluoroFinder tracks 93 commercially available iFluor 488 conjugates, searchable by target, clone, host and application.