Janelia Fluor 585
Janelia Fluor® 585 (Ex-Max 585 nm/Em-Max 609 nm)
Janelia Fluor® 585 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 585 has an excitation peak at 585 nm and an emission peak at 609 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 585 nm
- Emission maximum
- 609 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
- Relative brightness
- 5 of 5
- Extinction coefficient
- 156000
- Quantum yield
- 0.78
- Commercial antibodies
- 17.8k from 1 supplier
Janelia Fluor 585 has an excitation maximum at 585 nm and an emission maximum at 609 nm, a Stokes shift of 24 nm. Its extinction coefficient is 156000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Janelia Fluor 585?
- Janelia Fluor® 585 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 585 has an excitation peak at 585 nm and an emission peak at 609 nm.
- What laser excites Janelia Fluor 585?
- Janelia Fluor 585 is optimally excited by the 561 laser line. Its excitation maximum is 585 nm.
- What filter should I use to detect Janelia Fluor 585?
- A 610/20 filter captures the peak of the Janelia Fluor 585 emission near 609 nm with minimal spillover.
- How bright is Janelia Fluor 585?
- Janelia Fluor 585 has a relative brightness of 5 out of 5 (quantum yield 0.78).
- How many antibodies are available conjugated to Janelia Fluor 585?
- FluoroFinder tracks 17836 commercially available Janelia Fluor 585 conjugates, searchable by target, clone, host and application.