APC-eFluor 780
APC-eFluor™ 780 (Ex-Max 655 nm/Em-Max 780 nm)
APC-eFluor™ 780 (APC-eF780) is a red-emitting tandem fluorophore of APC, excited by the 640 nm red laser and transfers energy to the acceptor, eF780, which emits light that can be captured with a 780/60 nm bandpass filter. APC-eF780 has an excitation peak at 645 nm and an emission peak at 767 nm, and is spectrally similar to APC-CF®750 (Biotium), APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec) and APC-Fire™ 750 (BioLegend). This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
Excitation & emission spectra
Key facts
- Excitation maximum
- 655 nm
- Emission maximum
- 780 nm
- Optimal laser line
- 628-640
- Optimal filter
- 780/60
- Relative brightness
- 2 of 5
- Extinction coefficient
- 700000
- Molecular weight
- 105000
- Commercial antibodies
- 328 from 3 suppliers
APC-eFluor 780 has an excitation maximum at 655 nm and an emission maximum at 780 nm, a Stokes shift of 125 nm. Its extinction coefficient is 700000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is APC-eFluor 780?
- APC-eFluor™ 780 (APC-eF780) is a red-emitting tandem fluorophore of APC, excited by the 640 nm red laser and transfers energy to the acceptor, eF780, which emits light that can be captured with a 780/60 nm bandpass filter. APC-eF780 has an excitation peak at 645 nm and an emission peak at 767 nm, and is spectrally similar to APC-CF®750 (Biotium), APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec) and APC-Fire™ 750 (BioLegend). This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
- What laser excites APC-eFluor 780?
- APC-eFluor 780 is optimally excited by the 628-640 laser line. Its excitation maximum is 655 nm.
- What filter should I use to detect APC-eFluor 780?
- A 780/60 filter captures the peak of the APC-eFluor 780 emission near 780 nm with minimal spillover.
- How bright is APC-eFluor 780?
- APC-eFluor 780 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to APC-eFluor 780?
- FluoroFinder tracks 328 commercially available APC-eFluor 780 conjugates, searchable by target, clone, host and application.