Alexa Fluor 546
Alexa Fluor™ 546 (Ex-Max 556 nm/Em-Max 573 nm)
Alexa Fluor™ 546 (AF546, Alexa 546) has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor® 555 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 546 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 556 nm
- Emission maximum
- 573 nm
- Optimal laser line
- 532
- Optimal filter
- 575/36
- Relative brightness
- 2 of 5
- Extinction coefficient
- 104000
- Quantum yield
- 0.1
- Molecular weight
- 1080
- Commercial antibodies
- 53 from 4 suppliers
Alexa Fluor 546 has an excitation maximum at 556 nm and an emission maximum at 573 nm, a Stokes shift of 17 nm. Its extinction coefficient is 104000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Alexa Fluor 546?
- Alexa Fluor™ 546 (AF546, Alexa 546) has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor® 555 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 546 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
- What laser excites Alexa Fluor 546?
- Alexa Fluor 546 is optimally excited by the 532 laser line. Its excitation maximum is 556 nm.
- What filter should I use to detect Alexa Fluor 546?
- A 575/36 filter captures the peak of the Alexa Fluor 546 emission near 573 nm with minimal spillover.
- How bright is Alexa Fluor 546?
- Alexa Fluor 546 has a relative brightness of 2 out of 5 (quantum yield 0.1).
- How many antibodies are available conjugated to Alexa Fluor 546?
- FluoroFinder tracks 53 commercially available Alexa Fluor 546 conjugates, searchable by target, clone, host and application.