Qdot 545
Qdot 545 (Ex-Max 300 nm/Em-Max 545 nm)
Qdot® 545 from Thermo Fisher Scientific is an inorganic nanocrystal with a surface functionalized to allow antibodies and biomolecules to be cross-linked. It is most efficiently excited between 320-405 nm but is capable of excitation across every laser less than its emission peak. It is significantly brighter and more photostable than traditional organic fluorophores, making it still useful for fluorescence microscopy, however its size and solubility issues make it a challenge to use in flow cytometry. In spectral flow cytometry it may still prove useful due to its very unique spectral characteristics
Excitation & emission spectra
Key facts
- Excitation maximum
- 355 nm
- Emission maximum
- 545 nm
- Optimal laser line
- 355, 405
- Optimal filter
- 530/43
- Relative brightness
- 2 of 5
Qdot 545 has an excitation maximum at 355 nm and an emission maximum at 545 nm, a Stokes shift of 190 nm. It is optimally excited by the 355, 405 laser line.
Frequently asked questions
- What is Qdot 545?
- Qdot® 545 from Thermo Fisher Scientific is an inorganic nanocrystal with a surface functionalized to allow antibodies and biomolecules to be cross-linked. It is most efficiently excited between 320-405 nm but is capable of excitation across every laser less than its emission peak. It is significantly brighter and more photostable than traditional organic fluorophores, making it still useful for fluorescence microscopy, however its size and solubility issues make it a challenge to use in flow cytometry. In spectral flow cytometry it may still prove useful due to its very unique spectral characteristics
- What laser excites Qdot 545?
- Qdot 545 is optimally excited by the 355, 405 laser line. Its excitation maximum is 355 nm.
- What filter should I use to detect Qdot 545?
- A 530/43 filter captures the peak of the Qdot 545 emission near 545 nm with minimal spillover.
- How bright is Qdot 545?
- Qdot 545 has a relative brightness of 2 out of 5.