APC
APC (Ex-Max 651 nm/Em-Max 660 nm)
Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 651 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 5 of 5
- Extinction coefficient
- 700000
- Quantum yield
- 0.68
- Molecular weight
- 105000
- Commercial antibodies
- 81.6k from 56 suppliers
APC has an excitation maximum at 651 nm and an emission maximum at 660 nm, a Stokes shift of 9 nm. Its extinction coefficient is 700000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is APC?
- Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
- What laser excites APC?
- APC is optimally excited by the 628-640 laser line. Its excitation maximum is 651 nm.
- What filter should I use to detect APC?
- A 660/20 filter captures the peak of the APC emission near 660 nm with minimal spillover.
- How bright is APC?
- APC has a relative brightness of 5 out of 5 (quantum yield 0.68).
- How many antibodies are available conjugated to APC?
- FluoroFinder tracks 81597 commercially available APC conjugates, searchable by target, clone, host and application.